Regulatory

Part:BBa_K2924039:Design

Designed by: Katharina Polzen   Group: iGEM19_Duesseldorf   (2019-10-13)


AOX1 methanol-inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

. AOX1 is a strong, methanol-inducible promoter, which is not active in the presence of glucose.


Source

Pichia pastoris easy expression kit (Invitrogen)

References